Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay.
Identifieur interne : 002616 ( Main/Exploration ); précédent : 002615; suivant : 002617Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N(2)-dG adducts by capillary electrophoresis immunoassay.
Auteurs : Chao Wang [République populaire de Chine] ; Tao Li ; Zhixin Wang ; Feng Feng ; Hailin WangSource :
- Journal of chromatography. A [ 1873-3778 ] ; 2010.
Descripteurs français
- KwdFr :
- 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol (), 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol (analogues et dérivés), 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol (métabolisme), Adduits à l'ADN (métabolisme), Anticorps monoclonaux (métabolisme), Dosage immunologique (), Désoxyguanosine (), Désoxyguanosine (analogues et dérivés), Désoxyguanosine (métabolisme), Fixation compétitive, Fluorescence, Génome humain (génétique), Humains, Lasers, Lignée cellulaire tumorale, Modèles linéaires, Rhodamines (métabolisme), Stéréoisomérie, Électrophorèse capillaire ().
- MESH :
- analogues et dérivés : 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol, Désoxyguanosine.
- génétique : Génome humain.
- métabolisme : 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol, Adduits à l'ADN, Anticorps monoclonaux, Désoxyguanosine, Rhodamines.
- 7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol, Dosage immunologique, Désoxyguanosine, Fixation compétitive, Fluorescence, Humains, Lasers, Lignée cellulaire tumorale, Modèles linéaires, Stéréoisomérie, Électrophorèse capillaire.
English descriptors
- KwdEn :
- 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (analogs & derivatives), 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (chemistry), 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (metabolism), Antibodies, Monoclonal (metabolism), Binding, Competitive, Cell Line, Tumor, DNA Adducts (metabolism), Deoxyguanosine (analogs & derivatives), Deoxyguanosine (chemistry), Deoxyguanosine (metabolism), Electrophoresis, Capillary (methods), Fluorescence, Genome, Human (genetics), Humans, Immunoassay (methods), Lasers, Linear Models, Rhodamines (metabolism), Stereoisomerism.
- MESH :
- chemical , analogs & derivatives : 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, Deoxyguanosine.
- chemical , chemistry : 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, Deoxyguanosine.
- chemical , metabolism : 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, Antibodies, Monoclonal, DNA Adducts, Deoxyguanosine, Rhodamines.
- genetics : Genome, Human.
- methods : Electrophoresis, Capillary, Immunoassay.
- Binding, Competitive, Cell Line, Tumor, Fluorescence, Humans, Lasers, Linear Models, Stereoisomerism.
Abstract
The stereospecific binding of monoclonal antibody (mAb) 8E11 to anti-benzo(a)pyrene diol epoxide (BPDE)-dG adducts in single nucleoside, long oligonucleotide, and genomic DNA were quantitatively evaluated using noncompetitive and competitive capillary electrophoresis (CE) immunoassays. Two single-stranded TMR-BPDE-90 mers containing a single anti-BPDE-dG adduct with defined stereochemistry and a fluorescent label at 5'-end were used as fluorescent probes for competitive CE immunoassay. To quantitatively evaluate the binding affinity through competitive CE immunoassays, a series of equations were derived according to the binding stoichiometry. The binding of mAb 8E11 to trans-(+)-anti-BPDE-dG displays strongest affinity (K(b): 3.57 x 10(8) M(-1)) among all four investigated anti-BPDE-dG mononucleoside adducts, and the cis-(-)-anti-BPDE-dG displays lowest affinity (K(b): 1.14 x10(7) M(-1)). The binding of monoclonal antibody (mAb) 8E11 to BPDE-dG adducts in long DNA (90 mer) preferentially forms the complex with a stoichiometry of 1:1, and that mAb 8E11 displays a slightly higher affinity with trans-(+)-anti-BPDE-90 mers (K(b): 6.36+/-0.54 x 10(8)M(-1)) than trans-(-)-anti-BPDE-90 mers (K(b): 4.52+/-0.52 x 10(8) M(-1)). The mAb 8E11 also displays high affinity with BPDE-dG adducts in genomic DNA (K(b): 3.74 x 10(8) M(-1)), indicating its promising applications for sensitive immuno-detection of BPDE-DNA adducts in genomic DNA.
DOI: 10.1016/j.chroma.2010.02.024
PubMed: 20223464
Affiliations:
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Two single-stranded TMR-BPDE-90 mers containing a single anti-BPDE-dG adduct with defined stereochemistry and a fluorescent label at 5'-end were used as fluorescent probes for competitive CE immunoassay. To quantitatively evaluate the binding affinity through competitive CE immunoassays, a series of equations were derived according to the binding stoichiometry. The binding of mAb 8E11 to trans-(+)-anti-BPDE-dG displays strongest affinity (K(b): 3.57 x 10(8) M(-1)) among all four investigated anti-BPDE-dG mononucleoside adducts, and the cis-(-)-anti-BPDE-dG displays lowest affinity (K(b): 1.14 x10(7) M(-1)). The binding of monoclonal antibody (mAb) 8E11 to BPDE-dG adducts in long DNA (90 mer) preferentially forms the complex with a stoichiometry of 1:1, and that mAb 8E11 displays a slightly higher affinity with trans-(+)-anti-BPDE-90 mers (K(b): 6.36+/-0.54 x 10(8)M(-1)) than trans-(-)-anti-BPDE-90 mers (K(b): 4.52+/-0.52 x 10(8) M(-1)). The mAb 8E11 also displays high affinity with BPDE-dG adducts in genomic DNA (K(b): 3.74 x 10(8) M(-1)), indicating its promising applications for sensitive immuno-detection of BPDE-DNA adducts in genomic DNA.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Feng, Feng" sort="Feng, Feng" uniqKey="Feng F" first="Feng" last="Feng">Feng Feng</name><name sortKey="Li, Tao" sort="Li, Tao" uniqKey="Li T" first="Tao" last="Li">Tao Li</name><name sortKey="Wang, Hailin" sort="Wang, Hailin" uniqKey="Wang H" first="Hailin" last="Wang">Hailin Wang</name><name sortKey="Wang, Zhixin" sort="Wang, Zhixin" uniqKey="Wang Z" first="Zhixin" last="Wang">Zhixin Wang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Chao" sort="Wang, Chao" uniqKey="Wang C" first="Chao" last="Wang">Chao Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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